论文部分内容阅读
Objective:To explore the effect of emulsified isoflurane(EI)on apoptosis of anoxia-reoxygenation neonatal rat cardiomyocytea and relevant protein expression.Methods:Cardiac muscle anoxiareoxygenation damage model was established with culture in vitro neonatal rat cardiomyocytes.The cardiomyocytes were divided into control group,model group,fat emulsion group and EI group.The cardiomyocytes apoptosis rates and lactic dehydrogenase(LDH),superoxide dismutase(SOD)and malondialdehyde(MDA)index standardization were detected after relevant treatment The expression of apoptosis-related proteins Bel-2,Bax and Caspase-3 were detected with Western blot approach.Results:After hypoxia/reoxygenation(H/R)model was treated by EI,the cells apoptosis rate decreased and was dramatically below the fat emulsion group(P<0.05),Cardiomyocytes biochemical index detection presented that,compared with the control group that the LDH activity and MDA content dramatically increased(P<0.05),while the SOD activity notably decreased(P<0.05);compared with the H/R group,the SOD activity of the fat emulsion group and EI group increased(P<0.05);while the LDH activity and MDA content decreased(P<0.05).And the change of the EI group was more remarkable than the fat emulsion group(P<0.05).The Western blot analysis presented that,compared with the control group,the Bcl-2 protein expression of the other groups significantly decreased(P<0.05),the expressions of Bax protein and Caspase-3protein increased significantly(P<0.05);compared with H/R group,cardiomyocytes Bc1-2protein expression of EI group increased significantly(P<0.05),the expressions of Bax protein and Caspase-3 protein decreased significantly(P<0.05),and the change of EI group was more remarkable than the fat emulsion group(P<0.05).Conclusions:EI can inhabit the apoptosis of anoxia-reoxygenation damage model cardiomyocytes,and may he related to the up-regulation of expression of Bcl-2 and down-regulation of expression of Caspase-3 protein.
Objective: To explore the effect of emulsified isoflurane (EI) on apoptosis of anoxia-reoxygenation neonatal rat cardiomyocytea and relevant protein expression. Methods: Cardiac muscle anoxiareoxygenation damage model was established with culture in vitro neonatal rat cardiomyocytes. The cardiomyocytes were divided into control group , model group, fat emulsion group and EI group. The cardiomyocytes apoptosis rates and lactic dehydrogenase (LDH), superoxide dismutase (SOD) and malondialdehyde (MDA) index standardization were detected after relevant treatment The expression of apoptosis-related proteins Bel- Bax and Caspase-3 were detected with Western blot approach. Results: After hypoxia / reoxygenation (H / R) model was treated by EI, the cells apoptosis rate decreased and was dramatically below the fat emulsion group (P <0.05) index detection presented that, compared with the control group that the LDH activity and MDA content dramatically increased (P <0.05), while the SOD activity no Compared with the H / R group, the SOD activity of the fat emulsion group and EI group increased (P <0.05); while the LDH activity and MDA content decreased (P <0.05) .And the The results of the EI group was more remarkable than the fat emulsion group (P <0.05). Western blot analysis presented that, compared with the control group, the Bcl-2 protein expression of the other groups significantly decreased the expressions of Bax protein and Caspase-3 protein increased significantly (P <0.05); compared with H / R group, the expressions of Bax protein and Caspase-3 protein (P <0.05), and the change of EI group was more remarkable than the fat emulsion group (P <0.05) .Conclusions: EI can inhabit the apoptosis of anoxia-reoxygenation damage model cardiomyocytes, and may he related to the up -regulation of expression of Bcl-2 and down-regulation of expression of Caspase-3 protein.