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目的：研究创伤性损伤后大脑皮层细胞ＮＡＤＰＨ－ｄ组化反应变化情况，探讨继发性脑损伤的某些机制。方法：用机械划痕法创伤性损伤混合培养１０ｄ的大鼠大脑皮层神经细胞，采用ＮＡＤＰＨ－ｄ组化染色和ＮＳＥ免疫组化染色法观察神经元和胶质细胞中的一氧化氮（ＮＯ）产生情况。结果：正常培养皮层神经元中有少量ＮＡＤＰＨ－ｄ阳性反应神经元，创伤性损伤后阳性反应神经元数量增加；胶质细胞中有ＮＡＤＰＨ－ｄ阳性反应，在创伤性损伤混合培养皮层神经元创道周围，ＮＡＤＰＨ－ｄ阳性反应明显强于其它部位。结论：创伤可致神经元和胶质细胞过量产生和释放ＮＯ并在继发性脑损伤中可能具有重要意义。
Objective: To study the changes of NADPH-d histochemistry in cerebral cortex after traumatic injury and to explore some mechanisms of secondary brain injury. Methods: Nerve cells and nerve cells were stained with NADPH-d and NSE immunohistochemistry to observe the effects of nitric oxide (NO) on neurons and glial cells. Produce the situation. Results: There were a few NADPH-d-positive neurons in normal cultured cortical neurons. The number of positive neurons after traumatic injury increased. NADPH-d positive reaction was found in glial cells. In traumatic injury mixed cultured cortical neurons Road around, NADPH-d positive reaction was significantly stronger than the other parts. CONCLUSION: Trauma can cause overproduction of neurons and glial cells and release of NO, which may be of importance in secondary brain injury.