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目的探讨D-氨基葡萄糖衍生物2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖(COPADG)对人食管癌Eca-109细胞凋亡的影响及其作用机制。方法体外培养Eca-109细胞;倒置显微镜下观察细胞形态学变化;流式细胞术检测COPADG作用后Eca-109细胞内半胱氨酸酶3(caspase-3)活化程度及bcl-2蛋白表达的变化;Annexin V/PI染色结合流式细胞术检测COPADG作用后Eca-109细胞凋亡率。结果COPADG作用显著增加Eca-109细胞凋亡率,且Eca-109细胞内caspase-3活性和bcl-2表达平均荧光强度明显增高。结论COPADG能显著诱导Eca-109细胞凋亡发生,并使Eca-109细胞内caspase-3显著活化以及bcl-2蛋白表达下调,并可能通过该机制诱导细胞凋亡的发生。
Objective To investigate the effect of D-glucosamine derivative 2- (3-carboxy-1-propionylamino) -2-deoxy-D-glucose on the apoptosis of human esophageal cancer Eca-109 cells and its mechanism. Methods Eca-109 cells were cultured in vitro. Morphological changes of cells were observed under inverted microscope. The activation of caspase-3 and the expression of bcl-2 protein in Eca-109 cells were detected by flow cytometry The apoptotic rate of Eca-109 cells was detected by Annexin V / PI staining combined with flow cytometry. Results The COPADG treatment significantly increased the apoptosis rate of Eca-109 cells, and the average fluorescence intensity of caspase-3 activity and bcl-2 expression in Eca-109 cells was significantly increased. Conclusion COPADG can significantly induce the apoptosis of Eca-109 cells, activate caspase-3 and down-regulate the expression of bcl-2 in Eca-109 cells, and induce the apoptosis of Eca-109 cells.