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目的通过构建金黄色葡萄球菌(Staphylococcus aureus,简称金葡菌)中SarS蛋白突变株,研究SarS蛋白在金葡菌中的调控功能。方法利用同源重组的方法构建金葡菌8325-4来源的sarS插入突变菌株,通过MDBK细胞模型检测突变株外毒素水平,以及其在高盐、高温等条件下的生长情况。结果成功构建了金葡菌8325-4来源的sarS插入突变菌株。进一步的表型检测结果显示,与野生型菌株相比,sarS突变株在高温、高渗、重金属等应激条件下的生长速度明显下降,但是细菌外毒素对MDBK细胞的杀伤作用没有明显的改变。结论 SarS蛋白在金葡菌抵抗外界压力应激中发挥着重要的功能。
Objective To study the regulation of SarS protein in Staphylococcus aureus by constructing a SarS protein mutant in Staphylococcus aureus (Staphylococcus aureus). Methods The sarS insertion mutant strain of Staphylococcus aureus 8325-4 was constructed by homologous recombination. The exotoxin level of mutant strain was detected by MDBK cell model and its growth under high salt and high temperature conditions. Results The sarS insertion mutant strain of Staphylococcus aureus 8325-4 was successfully constructed. Further phenotypic analysis showed that the growth rate of sarS mutants was significantly lower than that of wild type strains under the stress of high temperature, hypertonic and heavy metals, but the killing effect of exotoxin on MDBK cells did not change significantly . Conclusion SarS protein plays an important role in Staphylococcus aureus resistance to external stress.