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目的：观察放烧复合伤小鼠骨髓基质细胞体外集落生成能力的变化。方法：采用Ｄｅｘｔｅｒ型骨髓基质细胞体外培养法，观察５．０Ｇｙγ线放射损伤、１５％Ⅲ度体表面积烧伤和放烧复合伤小鼠于伤后３、７、１４ｄ和经体外培养１４、２１、２８ｄ的骨髓基质细胞集落形成能力（ＣＦＵ－Ｆ）的变化。结果：①单放组和复合伤组均以伤后第７天最低，至伤后第１４ｄ仍未达正常组水平（Ｐ＜０．０１）；单烧组除伤后第３ｄ低于正常组外（Ｐ＜０．０５），至伤后第１４ｄ与正常组无显著差异。②伤后经不同培养时间的ＣＦＵ－Ｆ动态变化：在同一规格的培养皿中，正常组和单烧组的骨髓基质细胞均在培养２８ｄ时形成长满培养皿底的基质细胞层；单放组的骨髓基质细胞至培养２８ｄ时仍未长满培养皿底；复合伤组的ＣＦＵ－Ｆ形成能力高于单放组，但逊于正常组和单烧组。结论：单放组和复合伤组骨髓基质细胞受损后恢复缓慢；放烧复合伤对骨髓基质细胞的损伤具有放射损伤为主的特点。
OBJECTIVE: To observe the changes of colony-forming ability of bone marrow stromal cells in mice exposed to radii-bleratin. Methods: Dexter-type bone marrow stromal cells cultured in vitro were used to observe the effects of 5.0 Gy γ-ray radiation injury, 15% Ⅲ body surface burn and burn combined injury on the 3rd, 7th and 14th day after injury and 14,21, 28d bone marrow stromal cell colony forming ability (CFU-F) changes. Results: ① The lowest in the 7th day after injury was found in the group of unilateral radiotherapy and the group of the combined injury, but still not reached the level of the normal group on the 14th day after injury (P <0.01) (P <0.05), but there was no significant difference with the normal group on the 14th day after injury. (2) Dynamic changes of CFU-F in different culture time after injury: The bone marrow stromal cells in the normal group and the single-burn group all formed the stromal cell layer with the bottom of the petri dish at the 28th day after culture; The bone marrow stromal cells of the group did not grow to the bottom of the culture dish till 28 days of culture. The CFU-F formation ability of the combined injury group was higher than that of the normal group and the single burn group. Conclusion: Bone marrow stromal cells in group B and group B have a slow recovery after injury. The combination of burn and burn injury has the characteristics of radiation injury on the marrow stromal cells.