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氮素是植物生长发育所必需的大量元素,也是限制植物产量的首要因素,硝酸盐转运蛋白NRT是植物吸收和利用氮素的重要蛋白。笔者以香蕉为实验材料,通过RNA-Seq测序,筛选得到一个显著差异表达的高亲和硝酸盐转运蛋白基因NRT2;通过PCR克隆获得1 509 bp的c DNA序列,生物信息学预测其编码502氨基酸,含有MFS结构域,属于NRT2基因家族,命名为MaNRT2;RNA-Seq和qRT-PCR的结果显示,MaNRT2基因的表达具有显著的组织特异性,在根中远高于叶片;低氮胁迫处理后,MaNRT2在叶片中表达量上升,在根中表达量反而下降,表明MaNRT2与香蕉氮元素的吸收转运密切相关。
Nitrogen is a large number of elements necessary for plant growth and development, and is also the primary factor limiting plant yield. Nitrate transporter NRT is an important protein that plants use to absorb and utilize nitrogen. Using banana as the experimental material, we obtained a highly differentially expressed high-affinity nitrate transporter gene NRT2 by RNA-Seq sequencing. The cDNA sequence of 1 509 bp was obtained by PCR cloning. Bioinformatics predicts that it encodes 502 amino acids , Containing the MFS domain and belonging to the NRT2 gene family and named as MaNRT2. The results of RNA-Seq and qRT-PCR showed that the expression of MaNRT2 gene was significantly tissue-specific and much higher in roots than in leaves. After treatment with low nitrogen stress, The expression of MaNRT2 increased in leaves and decreased in roots, indicating that MaNRT2 was closely related to the absorption and transport of nitrogen.