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本试验研究了10种不同成份的培养基以及延长诱导培养时间对水稻成熟种胚培养力的影响。结果表明,水稻品种对不同培养基的反应是不同的。以∠S—5(∠S基本培养基+1.5mg/L2.4—D+1mg/1 KT+2%蔗糖+0.8%琼脂,pH5.8)培养基的愈伤组织诱导率为最高,诱导的愈伤组织质量较好。诱导的愈伤组织其绿苗分化率也较高,诱导培养60天其愈伤组织仍具有较高的分化能力。
In this study, the effects of 10 different mediums and prolonged induction of culture on the culture ability of rice seedlings were studied. The results showed that rice varieties responded differently to different media. The callus induction rate was the highest with ∠S-5 (∠S basal medium + 1.5mg / L2.4-D + 1mg / 1 KT + 2% sucrose + 0.8% agar, pH5.8) The callus quality is better. The induced callus also had a higher differentiation rate of green shoots, and the callus still had higher differentiation ability after 60 days induction culture.