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目的观察肝动脉As2O3碘油栓塞对兔肝移植瘤凋亡及增生细胞核抗原表达及微血管密度(MVD)的影响。方法32只家兔肝内肿瘤种植后2周,随机分4组,经肝动脉插管分别给予不同处理,实验设生理盐水灌注组(A组)、单纯碘油栓塞组(B组)、阿霉素碘油栓塞组(C组)及As2O3碘油栓塞组(D组)。治疗后1周,免疫组化方法测定肿瘤区的MVD值及增生细胞核抗原的表达,原位末端标记法检测肿瘤的凋亡指数。结果治疗后1周,单纯碘油栓塞及阿霉素碘油栓塞组,残余肿瘤区的MVD略有升高,与生理盐水对照组相比,统计学无显著性意义;As2O3碘油栓塞组残余瘤区MVD减低,与其他组相比差异具有显著性意义。各组凋亡指数和增殖指数分别为1.53±0.42、2.66±0.54、2.91±0.32、3.44±0.65和60.8±15.5、42.4±11.2、40.6±8.8、28.5±5.7,两者存在负相关。结论As2O3碘油栓塞可以通过诱导肿瘤细胞凋亡,抑制肿瘤细胞增生发挥抗肿瘤效应,As2O3碘油栓塞可以抑制残余肿瘤的血管新生。
Objective To observe the effect of hepatic arterial As2O3 lipiodol embolization on apoptosis and expression of proliferating cell nuclear antigen (PCNA) and microvessel density (MVD) in liver xenografts in rabbits. Methods Twenty-two rabbits were randomly divided into 4 groups after 2 weeks of implantation. Hepatic arterial cannulation was used to treat the intrahepatic tumor. The rats in each group were given normal saline (group A) and simple lipiodol (group B) (C group) and As2O3 lipiodol embolization group (D group). One week after the treatment, the MVD value and the expression of proliferating cell nuclear antigen in the tumor area were determined by immunohistochemistry. The apoptotic index of tumor was detected by in situ end labeling. Results One week after the treatment, the MVD in the residual tumor area increased slightly with lipiodol embolism alone and with doxorubicin lipiodol embolization group, which was not statistically significant compared with the saline control group. Tumor area MVD decreased, compared with other groups, the difference was significant. The apoptotic index and proliferation index in each group were 1.53 ± 0.42,2.66 ± 0.54,2.91 ± 0.32,3.44 ± 0.65 and 60.8 ± 15.5,42.4 ± 11.2,40.6 ± 8.8,28.5 ± 5.7 respectively. There was a negative correlation between them. Conclusion As2O3 lipiodol embolization can induce tumor cell apoptosis and inhibit tumor cell proliferation to exert anti-tumor effect. As2O3 lipiodol embolization can inhibit angiogenesis of residual tumor.