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目的 探讨神经肽YY2 (NPYY2 )受体调节大鼠学习记忆的可能机制。方法 建立学习记忆障碍模型 ,双侧海马插管注射Y2特异性激动剂NPY 3 36 ,观察其对大鼠学习记忆的影响 ,并利用免疫组化方法 ,检测与学习记忆密切相关的即刻早期基因c -fos表达情况。结果 1.注射NPY3 36组大鼠训练期的错误次数较对照组无显著性差别 (P >0 .0 5 ) ,而记忆测试期的潜伏期较对照组显著延长 ,错误次数显著减少 (P <0 .0 5 )。 2 .注射NPY 3 36组大鼠双侧背海马各区c -fos阳性细胞数与生理盐水组比较明显增加。结论 NPY通过Y2受体能促进大鼠的记忆能力 ,并可能通过促进c -fos基因上调参与大鼠的记忆形成过程
Objective To investigate the possible mechanism by which neuropeptide YY2 (NPYY2) receptors regulate learning and memory in rats. Methods The model of learning and memory impairment was established. The bilateral hippocampal intubation was injected with NPY 3 36, a Y2 specific agonist, to observe its effect on learning and memory in rats. Immunohistochemistry was used to detect immediate early gene c -fos expression. Compared with the control group, the number of mistakes in NPY3 36 injection group was not significantly different from that in the control group (P> 0.05), but the incubation period in memory test was significantly longer than that in the control group (P <0 .0 5). The number of c-fos positive cells in the bilateral hippocampus of NPY 36 rats was significantly increased compared with the saline group. Conclusion NPY can promote the memory ability of rats through Y2 receptor and may play a role in the memory formation of rats by promoting the up-regulation of c-fos gene