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目的为了探讨针刺镇痛效应与细胞免疫的关系,研究了针刺对小鼠巨噬细胞中c-fos mRNA,ppENKmRNA,iNOSmRNA及iNOS活性的效应.方法20只BALB/c小鼠被随机分成2组:a.用5Hz电针处理的针刺组;b.未用电针处理的对照组.针刺或牵拉前后用钾离子渗透法检测痛阈.实验采用原位杂交,NADPHNBT组织化学,RNA斑点印迹及蛋白质斑点印迹技术.应用TLC扫描仪扫描斑点印迹信号并作统计学处理.结果杂交信号和iNOS活性呈现蓝紫色,信号均定位于巨噬细胞的胞质.与对照组相比,针刺组的所有印迹信号均增强,P＜0.01.针刺组中c-fos mRNA,ppENKmRNA,iNOSmRNA的表达及iNOS的活性与提高的痛阈呈正相关.此外,c-fos mRNA与ppENKmRNA之间的变动呈正相关.结论针刺可上调小鼠巨噬细胞中c-fos,ppENK,iNOS的基因表达,且提示c-fos可能参与ppENK基因表达,c-fosmRNA与ppENKmRNA可能同时表达.“,”Objective To investigate the relationship between acupuncture analgesia and cellular immunity, the acupuncture effect on expression of c-fos mRNA, ppENKmRNA,iNOSmRNA and iNOS activity in the mouse peritoneal macrophage were studied. Methods Twenty BALB/c mice were randomly divided into 2 groups:a, the acupuncture group treated with 5Hz electroacupuncture (EA); b, the control group treated with no EA.The pain threshold was detected by K+ ionophoresis method before and after EA or restraining. The experiment was performed by using in situ hybridization, NADPH-NBT histochemistry, RNA dot blot and protein dot blot techniques. All dot blot signals were scanned for statistical analysis. Results The hybridization signals and iNOS activity appeared as granules in bluish violet color, localized in the cytoplasm of the macrophage. All dot blot signals were increased in the acupuncture group, when compared with those in the control group(P＜0. 01 ). Besides,there was a positive correlation in alteration between the analgesic effect and all the signals, and between the c-fos mRNA signals and ppENKmRNA signals. Conclusion Acupuncture could induce analgesic effect and gene expression of c-fos mRNA, ppENKmRNA and iNOS with positive correlation in the peritoneal macrophage, suggesting that acupuncture could promote cellular immunity through neuroimmunological pathway. The positive correlation in alteration between the c-fos mRNA signals and ppENKmRNA signals with similar intensity suggests that the cfos may involve gene expression of ppENK and both genes may express about at the same time in the macrophage.