论文部分内容阅读
本文采用合成交叠肽扫描的方法在６种不同Ｈ－２单倍型小鼠确定了ｄ／ｙ二种亚型Ｐｒｅ－Ｓ＿（２）蛋白的Ｔ细胞识别部位。发现：（１）在所观察的６种同类系小鼠均存在对Ｐｒｅ－Ｓ＿（２）蛋白反应的亚型特异性，这可能与Ｐｒｅ－Ｓ＿（２）蛋白分子Ｃ末端的多态性有关；（２）Ｐｒｅ－Ｓ＿（２）蛋白的Ｔ细胞识别部位在５种同类系小鼠位于Ｃ末端３２个氨基酸残基顺序内，在另一种同类系小鼠则位于Ｎ末端顺序，这与我们先前的理论观测结果相一致；（３）进一步分析发现：不同Ｈ２单倍型小鼠对Ｐｒｅ－Ｓ＿（２）蛋白的Ｔ细胞识别是ＭＨＣ分子依赖的；（４）２４－５５肽段的圆二色性表明该肽段内存在一个α－螺旋，可能是其作为Ｔ细胞识别部位的结构基础。以上结果对于设计新一代乙型肝炎疫苗有意义。
In this paper, T-cell recognition sites of Pre-S_ (2) protein of d / y subtypes were identified in six different H-2 haplotypes using a synthetic overlapping peptide scanning method. The results showed that: (1) The subtype specificity of Pre-S_ (2) protein response existed in all the 6 kinds of mice observed, which may be related to the C-terminal polymorphism of Pre-S_ (2) ; (2) The T-cell recognition site of Pre-S_ (2) protein is located in the C-terminal 32 amino acid residues of five congenic mice and in the N-terminal sequence of another mouse of the same family, Our previous theoretical observations are consistent; (3) Further analysis found that: different H2 haplotype mice T-cell recognition Pre-S_ (2) protein MHC molecule-dependent; (4) 24-55 peptide Circular dichroism indicates the presence of an alpha-helix in this peptide, probably as a structural basis for the recognition site of T cells. The above results are meaningful for designing a new generation of hepatitis B vaccine.