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(1) 在陝西关中所采的黄萎病病株棉籽內部,常带有一些真菌和細菌,虽經用浓硫酸脫絨,这些菌类仍能从棉籽本身的組織內向培养基伸展惺庇玫捅剁R直接检查虽亦能見到輪枝菌从棉籽內露出,但由于其他菌类的生长速度較快,以致輪枝菌常被干扰,无从在洋菜上发展,故用一般方法很难分离和检查到棉籽所带的輪枝菌。(2) 用不含养分的水洋菜作为培养基时,杂菌的生长減弱,轮枝菌才能在基物上发展为菌落。又用流水冲洗法代替表面消毒,使棉籽吸收水分,同时也促使潛伏菌絲继續发展,并可冲去細菌。經試驗用上述两个步驟可以使棉籽所带的輪枝菌在水洋菜上形成菌落。結合低倍鏡直接检查法,可以較为方便地检查种籽带菌率。(3) 經初步拟定了一套检查方法和单孢子菌种分离法。惟应用时尚觉不够簡便,尚有待于进一步提高,特提出以供交流經驗。(4) 在1955—1957年的多次检查中,查得黄萎病病株棉籽带輪枝菌率为39.8%、5.9%及23.7%。在1958年初对大田种子进行检查,查得华阴等四县8个样品中带菌率为3%至23%,平均为12.3%。(5) 根据棉籽解剖培养結果,轮枝菌不仅存在于棉籽外部的短絨內,并且也存在于籽壳及籽仁上。(6) 从棉籽上分离到的轮枝菌特8号經初步鉴定系Verticillium albo-atrum Reinkeet Berth。(7) 特8号菌种經过接种試驗,确定其具有一定的致病力,并从再分离中获得該菌。(8) 棉籽是否能够传播黄萎病,似系一个检查方法問題。还希各方面进行测定,通过多次的检查和接种試驗始能充分确定。(9) 在分离的菌种中,除特8号菌种具有較深的菌落色泽外,还有一些菌种的顏色較淡,未須鑑定。棉籽所带的轮枝菌类型不一,还有进一步研究的必要。此外,棉籽內的輪枝菌和其他一些菌类究竟如何进入籽內,也有研究的价值。
(1) There are some fungi and bacteria in the cottonseed of Verticillium wilt disease strain in Guanzhong, Shaanxi Province. Although the fungus and bacteria are often removed by concentrated sulfuric acid, these fungi can still extend to the culture medium from the tissue of cotton seed itself Although Romyces Chopsticks R can also be seen directly from the cottonseed, it is hard to find that Verticillium is exposed to the cottonseed. However, due to the rapid growth of other fungi, Isolate and examine the rotten fungus that comes with cottonseed. (2) With nutrient-free Aquilegia as a medium, the growth of the bacteria is weakened, and Verticillium can develop into a colony on the substrate. Instead of running water disinfection instead of surface disinfection, cottonseed moisture absorption, but also to promote the continued development of latent mycelium, and can be washed away bacteria. After the test with the above two steps can make the cottonseed brought by the Verticillium in Aquilegia colony. Combined with low magnification direct inspection method, you can more easily check the seed carrier rate. (3) A set of inspection methods and single spore isolation method have been initially developed. However, the application of fashion is not easy enough, yet to be further improved, especially for the exchange of experience. (4) In the multiple inspections from 1955 to 1957, the prevalence of Verticillium wilt of Verticillium wilt strains was 39.8%, 5.9% and 23.7%, respectively. In early 1958, seeds of Daejeon were inspected. Among the eight samples in Huayin and other four counties, the inoculation rates were 3% to 23% with an average of 12.3%. (5) According to the result of cottonseed dissection, Verticillium is not only found in the short fiber outside the cottonseed, but also in the shell and the seed kernel. (6) Verticillium albo-atrum Reinkeet Berth. (7) The special strain No. 8 after inoculation test to determine its certain pathogenicity, and obtain the bacteria from the re-separation. (8) Whether cottonseed can transmit Verticillium wilt may be a problem of inspection method. Also hope all aspects of the determination, through a number of tests and inoculation test can be fully determined. (9) In the isolated strains, in addition to special No. 8 strains with dark colony color, there are some strains of lighter color, no identification. The types of rotifers that cottonseeds carry are different, and there is a need for further research. In addition, cottonseed within the Verticillium and some other fungi how to enter the seed, but also research value.