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目的研究均相酶免疫法检测甘胆酸(CG)在肝胆疾病中的临床应用。方法评估均相酶免疫法检测甘胆酸性能并与放射免疫法比对。随机选取2016年4月就诊安徽某医院患者308例,包括谷丙转氨酶(ALT)异常组88例,谷氨酰转移酶(GGT)异常组65例,总胆汁酸(TBA)异常组45例,总胆红素(TBIL)异常组80例,正常对照组30例,测定所有标本血清CG、TBIL、TBA、GGT、ALT、碱性磷酸酶(ALP),比较各异常组与正常对照组CG,分析每组CG与以上指标相关性。结果该法百分偏倚为3.47%~9.90%,批内精密度为3.25%~4.93%,批间精密度为5.46%~7.99%,CG参考区间为1.53μg/ml~1.81μg/ml;与放射免疫法相关性较好(P<0.05);各异常组与正常对照组CG差异有统计学意义(P<0.05);CG与TBA相关性较好(P<0.01),与GGT、TBIL、ALT、ALP相关性一般。结论均相酶免疫法检测甘胆酸在肝胆疾病中有较好应用价值。
Objective To study the clinical application of homogeneous enzyme immunoassay in the detection of glycocholic acid (CG) in hepatobiliary diseases. Methods The homogeneous enzyme immunoassay was used to evaluate the glycocholic acid and compared with radioimmunoassay. A total of 308 patients were randomly selected from a hospital in Anhui Province in April 2016, including 88 patients with abnormal ALT group, 65 patients with GGT abnormal group, 45 patients with abnormal total BA group, The serum levels of CG, TBIL, TBA, GGT, ALT and ALP were measured in all the patients with TBIL abnormalities and 30 normal controls. CG, Analysis of each group of CG and the above indicators relevance. Results The results showed that the method had a bias of 3.47% -9.90%, intra-assay precision of 3.25% -4.93%, inter-batch precision of 5.46% -7.99% and CG reference interval of 1.53μg / ml ~ 1.81μg / ml. The correlation between CG and TBA was better (P <0.01), and the correlation between CG and TBA was better (P <0.05), and there was significant difference between GGT, TBIL, ALT, ALP correlation in general. Conclusions The method of homogeneous enzyme immunoassay for the detection of glycocholate in hepatobiliary diseases has good value.