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用Ｃｕ￣（２＋）引发ＬＤＬ的氧化修饰，用ＦＯＸ法测定脂氢过氧化物（ＬＯＯＨ），观察了ＬＤＬ氧化修饰过程中ＬＯＯＨ的变化及外源性抗氧化剂维生素Ｅ和Ｃ的作用。结果显示ＬＤＬ氧化过程中ＬＯＯＨ的变化可分为三个阶段：首先ＬＯＯＨ有一段稳定不变的时间（延迟期），接着ＬＯＯＨ急剧上升（扩增期）；最后达到最大值并开始呈现下降趋势（降解期）。根据ＬＯＯＨ变化的动力学曲线可以求出ＬＤＬ氧化修饰的如下参数：ＬＯＯＨ基础值；延迟期时间；ＬＤＬ最大氧化速率和ＬＯＯＨ最大值。外源抗氧化剂维生素Ｅ和Ｃ的加入，可使延迟期的时间延长，但不影响ＬＯＯＨ的基础值、最大值和氧化速率。
Oxidative modification of LDL was induced by Cu ~ (2+). LOOH was measured by FOX method. The changes of LOOH and the effects of exogenous antioxidants such as vitamin E and C on the oxidative modification of LDL were observed. The results showed that the changes of LOOH during LDL oxidation can be divided into three stages: firstly, LOOH has a stable time (delay period), then LOOH increases sharply (amplification period); finally reaches the maximum value and begins to show a downward trend Degradation period). According to the kinetic curve of LOOH changes, the following parameters of LDL oxidative modification can be obtained: LOOH basal value, delay time, maximum LDL oxidation rate and maximum LOOH. The exogenous antioxidants vitamin E and C can prolong the delay period without affecting the basic value, maximum value and oxidation rate of LOOH.