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目的:分析血细胞分析仪测定血小板计数的主要影响因素。方法:选取该院2015年1~7月行健康体检的50例志愿者,分别采集其静脉血与末梢血行血小板计数测定,同时依次采用手工法与仪器法完成检测,再将不同时间测定血小板计数值进行比较,总结上述3种不同情况对检验结果的影响,并评估血细胞分析仪测定血小板计数的主要影响因素。结果:50例健康志愿者的静脉血标本血小板计数测定结果为(164.87±5.68)×10~9/L,末梢血标本测定结果为(162.19±5.14)×109/L,组间差异无统计学意义(P<0.05)。手工法与仪器法在采集即刻及静置8 h的血小板计数测定结果比较差异均无统计学意义(P<0.05),仪器法检验中静置10 min、1 h、2 h与4 h的血小板计数测定结果同采集即刻与静置8 h结果比较差异具有统计学意义(P<0.05)。结论:临床检验血小板计数时需严密关注采集部位与标本放置时间等因素,经全自动细胞分析仪测定血小板异常时,可酌情考虑手工计数法的应用,以避免血小板计数测定误差的发生。
Objective: To analyze the main influencing factors of platelet count by hematology analyzer. Methods: Fifty volunteers from January to July 2015 in our hospital for physical examination were enrolled. Blood samples were collected for determination of platelet count in venous and peripheral blood. Meanwhile, manual and instrumental methods were used to determine the platelet count. The platelet count Values were compared to summarize the above three different conditions on the test results, and to assess the main factors that determine the platelet count by the hematology analyzer. Results: The platelet count of the venous blood of 50 healthy volunteers was (164.87 ± 5.68) × 10 ~ 9 / L, and the peripheral blood samples were (162.19 ± 5.14) × 109 / L with no statistical difference Significance (P <0.05). There was no significant difference between the manual method and the instrumental method in the determination of platelet count immediately after collection and standing for 8 h (P <0.05). In the instrumental method, the platelets for 10 min, 1 h, 2 h and 4 h The result of counting was statistically different from that of collecting 8 h after standing and standing (P <0.05). CONCLUSIONS: In clinical tests of platelet counts, careful attention should be paid to factors such as collection site and specimen placement time. When automatic platelet analyzer detects platelet abnormalities, the manual counting method may be considered as appropriate to avoid the occurrence of platelet count error.