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甘草(Glycyrrhiza uralensis)种子搭载于一返回式卫星上,18天后返回地球(飞行回收舱平均辐射剂量为0.102mGy/d,飞行远地点距地球350km,重力为10?6×g),种子萌发并发育成熟.地面对照种子在相同环境下种植.甘草叶子用作ISSR分析,甘草一年生根的两种主要次生代谢产物采用高效液相色谱HPLC进行分析.在此实验中,使用22条引物,其中6条产生了不同基因条带.HPLC分析结果表明,搭载种子发育成熟的根的甘草酸(GA)和甘草苷(LQ)的含量分别比对照组高2.19倍和1.18倍.实验表明空间环境对甘草产生诱变作用并且影响其次生代谢产物.这些变化表明空间育种是一种新的有效方法并且能对濒危药用植物甘草资源的保护做出贡献.
The seeds of Glycyrrhiza uralensis were carried on a return satellite and returned to Earth after 18 days (average radiation dose in the recovery capsule was 0.102 mGy / d, distance from the earth to the earth was 350 km and the gravitational force was 10-6 × g), and the seeds germinated and developed Mature ground control seeds were planted under the same conditions Licorice leaves were used for ISSR analysis and the two major secondary metabolites of one year old root of licorice were analyzed by high performance liquid chromatography (HPLC) .In this experiment, 22 primers were used, of which 6 The results of HPLC showed that the contents of glycyrrhizic acid (GA) and glycyrrhizin (LQ) were 2.19 times and 1.18 times higher than that of the control group, respectively.Experiments showed that the space environment of licorice Produce mutagenic effects and affect their secondary metabolites.These changes indicate that space breeding is a new and effective method and can contribute to the protection of licorice resources of endangered medicinal plants.