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应用AFLP指纹技术对双孢蘑菇的 2 0个野生菌株和 5个栽培品种的遗传多样性进行了研究。AFLP指纹揭示出 2 0个野生异核体菌株所固有的基因型。 5个商业品种表现出比较一致的AFLP指纹 ,但也显示出它们之间的一些差别。由单孢分离获得的同核体菌株携带着部分异核体菌株的AFLP指纹 ;由同一子实体分离得到的大部分单孢菌株是异核体菌株 ,它们具有与其亲本一致的AFLP指纹。UPGMA分析揭示出 2个与地理分布 (美洲、欧洲 )相对应的组。研究结果表明 :(1 )在野生菌株之间存在着明显的遗传差异 ;(2 )大多数单孢分离的菌株具有与母本一致的遗传物质 ;(3)野生菌株间的遗传变异大于栽培品种间的变异 ;(4)在双孢蘑菇的遗传多样性分析中 ,AFLP技术是非常有效的工具
The genetic diversity of 20 wild strains and 5 cultivars of Agaricus bisporus was studied using AFLP fingerprinting. AFLP fingerprinting revealed genotypes native to 20 wild heterokaryotic strains. Five commercial varieties showed more consistent AFLP fingerprints, but also showed some differences between them. Isoenzyme strains obtained by single spore isolation carry AFLP fingerprints of some heteronuclear strains. Most of the isolates of Monospore isolated from the same fruiting body are heteronuclear strains with AFLP fingerprinting consistent with their parents. UPGMA analysis revealed two groups corresponding to geographical distribution (Americas, Europe). The results showed that: (1) there was a significant genetic difference between wild strains; (2) most of the single-spore isolates had the same genetic material as the female parent; (3) the genetic variation among the wild strains was greater than that of the cultivated ones (4) AFLP is a very effective tool in the genetic diversity analysis of Agaricus bisporus