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目的:研究姜辣素辅助昂丹司琼对顺铂所致大鼠急性期和延迟期异嗜行为的抑制作用.方法:大鼠随机分成正常对照组、模型组、昂丹司琼组、姜辣素组及姜辣素+昂丹司琼组,记录顺铂注射后0-24h和48-72h各组大鼠摄食量及啃食高岭土量.72h后取延髓极后区及近端空肠组织,免疫组织化学方法测定组织中P物质表达.结果:顺铂注射后0-24h和48-72h内,姜辣素组、昂丹司琼+姜辣素组摄食量较模型组显著增加(t=13.333,15.000,13.8333,15.167,均P<0.01),啃食高岭土量显著减少(t=9.122,12.986,8.525,8.902,均P<0.01);姜辣素组和昂丹司琼+姜辣素组摄食量均显著高于昂丹司琼组(t=8.000,9.667,11.500,12.833,均P<0.01);昂丹司琼+姜辣素组啃食高岭土量显著低于昂丹司琼组(t=2.499,3.999,均P<0.01);姜辣素组啃食高岭土量在0-24h内高于昂丹司琼组(t=1.365,P<0.05),48-72h内低于昂丹司琼组(t=3.622,P<0.01).昂丹司琼+姜辣素组在0-24h内啃食高岭土量显著低于姜辣素组(t=3.865,P<0.01),在48-72h内啃食高岭土量及摄食量与姜辣素组均无显著差异(t=0.377,1.333,均P>0.05).姜辣素组及昂丹司琼+姜辣素组的AP区P物质免疫组织化学表达均少于昂丹司琼组(t=2.217,2.408,均P<0.01).结论:姜辣素具有可促进大鼠摄食并可抑制顺铂所致的异嗜作用,与昂丹司琼联用可有效抑制急性期和延迟期异嗜行为,其机制可能与抑制P物质表达有关.
Objective: To study the inhibitory effect of gingerol supplemented ondansetron on the inhibition of cisplatin-induced acute and late metaphase in rats.Methods: The rats were randomly divided into normal control group, model group, ondansetron group, ginger In the group of spicy and gingerol + ondansetron, the rats’ food intake and the amount of grazing kaolin at 0-24h and 48-72h after cisplatin injection were recorded, and the posterior region of the medulla oblongata and proximal jejunum , And immunohistochemistry was used to detect the expression of substance P in the tissue.Results: In 0-24h and 48-72h after cisplatin injection, the food intake of the group of gingerol and ondansetron + gingerol was significantly increased (t = 13.333,15.000,13.8333,15.167, all P <0.01), the amount of grazing kaolin decreased significantly (t = 9.122,12.986,8.525,8.902, all P <0.01); the group of gingerol and ondansetron + ginger spicy (T = 8.000,9.667,11.500,12.833, all P <0.01), while the amount of eating kaolin in ondansetron + gingerol group was significantly lower than that of ondansetron (T = 2.499, 3.999, all P <0.01). The amount of grafted kaolin in gingerol group was higher than that in ondansetron group within 0-24h (t = 1.365, P <0.05) Ondansetron group (t = 3.622, P <0.01), ondansetron + gingerol group at 0-24h The content of grazing kaolin in ginkgolide group was significantly lower than that in the control group (t = 3.865, P <0.01). There was no significant difference in the amount of grazing kaolin and food intake between 48-72h and gingerol groups (t = 0.377,1.333, (All P> 0.05) .The immunohistochemical expression of substance P in the areas of the amlodipine and the ondansetron + gingerol groups was less than that of the ondansetron group (t = 2.217,2.408, both P <0.01). CONCLUSION: Gingerol can promote heterotrophic effects induced by cisplatin by ingestion of rats and its combination with ondansetron can inhibit acute and delayed heterophile effectively, and its mechanism may be related to inhibition of substance P expression related.