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目的 建立一种简便、直观的检测血吸虫循环抗原的方法。方法 以N 琥珀酰亚胺基 3 (2 吡啶基二硫 )丙酸酯为交联剂制备了抗血吸虫单克隆抗体与抗人“B”型红细胞单克隆抗体的双特异性嵌合抗体 ,并建立了以人“B”型红细胞为标志物的斑点红细胞免疫技术 (Dot RCIA)用以检测血吸虫抗原。结果 初步应用于检测血吸虫循环抗原表明 ,本方法具有较好的检测效果 ,同时方法简单、可肉眼判读结果。结论 本方法在检测血吸虫循环抗原方面具有较好的应用潜能
Objective To establish a simple and intuitive method for detecting circulating antigens of schistosoma japonicum. Methods Bispecific chimeric antibodies against human “B” erythrocyte monoclonal antibodies were prepared by using N-succinimidyl 3 (2-pyridyldithio) propionate as cross-linking agent. A Dot RCIA with human “B” type erythrocytes as a marker was developed to detect schistosome antigen. The results were initially applied to detect circulating antigens of Schistosoma japonicum, indicating that this method has good detection effect and that the method is simple and can be read by the naked eye. Conclusion The method has good potential in detecting circulating antigens of Schistosoma japonicum