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用Southern印迹或其它敏感的方法能在大多数尖锐湿疣皮损中检测到HPV DNA,而人类乳头瘤病毒(HPV)的主要衣壳蛋白(L1蛋白)检出的百分率范围从低于5％至高达80％。作者曾用免疫组化法检测尖锐湿疣患者皮损中L1蛋白,其百分率在妊娠妇女较非妊娠妇女或男性患者高。这可能与妊娠期细胞免疫改变、激素水平改变或其它在妊娠期尚未确定的因素有关。为了观察尖锐湿疣的解剖部位对L1蛋白检测的影响,作者用免疫组化技术检测了男性和非妊娠女性病损标本。用简单的记数方法确定L1染色阳性的核数量。
HPV DNA can be detected in most genital warts lesions by Southern blot or other sensitive methods whereas the percentage of major capsid protein (L1 protein) detected by human papilloma virus (HPV) ranges from less than 5% Up to 80%. The authors used immunohistochemistry to detect the L1 protein in patients with condyloma acuminata, the percentage of which was higher in pregnant women than in non-pregnant women or men. This may be related to changes in cellular immunity during pregnancy, changes in hormone levels or other factors not yet established during pregnancy. In order to observe the effect of anatomy of condyloma acuminatum on the detection of L1 protein, we used immunohistochemistry to examine lesion specimens of both male and nonpregnant women. A simple counting method was used to determine the number of L1-stained nuclei.