运动对高血压性肥大心脏心肌肌球蛋白重链基因表达的影响

• 作 者: 李昭波 高云秋 唐朝枢
• 机 构: 北京医科大学第三医院运动医学研究所!北京100083,北京医科大学第三医院运动医学研究所!北京100083,北京医科大学第三医院心血管基础研究所!北京100083
• 出 处: 中国应用生理学杂志
• 发表日期: 1999年04期
• 关键词: 高血压性 基因表达 肌球蛋白重链 大鼠 基因转录水平 分子杂交 同型 颈总动脉插管 左心室 图象分析系统

研究运动逆转高血压肥大心脏心肌肌球蛋白重链（ｍｙｏｓｉｎ ｈｅａｖｙ ｃｈａｉｎ ，ＭＨＣ）异型改变的分子机制。方法：采用Ｎｏｒｔｈｅｒｎ 分子杂交方法对自发性高血压大鼠（ｓｐｏｎｔａｎｅｏｕｓｌｙ ｈｙｐｅｒｔｅｎｓｉｖｅ ｒａｔｓ，ＳＨＲ） 游泳运动１０ 周后心肌ＭＨＣ基因表达进行比较研究。结果：游泳ＳＨＲ收缩压和舒张压分别比安静ＳＨＲ 降低２２ ％ 和２５％ （Ｐ＜ ０．０１） 。左心室重／体重（ＬＶＷ／ＢＷ）比值两组间无明显差异（Ｐ＞ ０．０５）。游泳ＳＨＲ 心肌αＭＨＣｍＲＮＡ表达比安静ＳＨＲ增强１７％ ，βＭＨＣｍＲＮＡ 表达降低２６ ％ ，α／βＭＨＣ基因表达比值提高５９ ％ 。结论：运动逆转高血压肥大心脏心肌ＭＨＣ同型异构体转变的调控机制可发生在基因转录水平上

To investigate the molecular mechanisms of exercise reversal of atypical changes in hypertrophic hypertensive cardiac myosin heavy chain (MHC). Methods: The MHC gene expression of myocardium in spontaneously hypertensive rats (SHR) swimming 10 weeks was compared by Northern hybridization. Results: The systolic and diastolic blood pressures of swimming SHR decreased by 22% and 25%, respectively (P <0.01), compared with that of resting SHR. Left ventricular mass / body weight (LVW / BW) ratio was no significant difference between the two groups (P> 0.05). The expression of αMHC mRNA in SHR myocardium was increased by 17% compared with that of resting SHR, the expression of βMHC mRNA was reduced by 26%, and the ratio of α / βMHC gene expression was increased by 59%. CONCLUSION: The mechanism by which exercise can reverse the MHC isoform transition in cardiac hypertrophy can occur at the gene transcription level