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目的:采用食物的咸度与热度,创建快速建立萎缩性胃炎动物模型的新方法。 方法:采用7周龄健康、性成熟的(?)SD大鼠,体质量200-250g共96只,按随机化原则分为6组,即正常喂养组(正常喂养,饮白开水)、正常对照组(25℃白开水灌胃,每日1次,每次2.5mL)、盐水组(25℃ 150g/L氯化钠灌胃,每日1次,每次2.5mL)、热水组(55℃蒸馏水灌胃,每日1次,每次2/5mL)、热盐水-Ⅰ组、(55℃150g/L氯化钠灌胃,每日1次,每次2.5ml)、热盐水-Ⅱ组(55℃ 150g/L氯化钠灌胃,每日2次,每次2.5ml)。每组各16只,采用架式笼养,恒温24±1℃,湿度50-60％。实验开始,先处死4只正常组大鼠,并留取胃大体标本作为对照。以后各组每2wk处死2只,以观察黏膜萎缩情况,造模成功(出现萎缩)即终止试验,留取胃黏膜标本。组织切片由专人采取盲法阅片。萎缩性胃炎标准的制定参照1994年美国休斯顿胃炎诊断分类标准及2000年井冈山胃炎分类标准,采用显微测微器对黏膜厚度、腺体宽度及细胞厚度进行测量。统计学处理:采用t检验。 结果:正常胃黏膜表面光滑,无糜烂,固有层无炎细胞浸润,黏膜下层无水肿,肌层无炎细胞浸润。热盐水灌胃至10wk,大鼠胃黏膜出现萎缩,热盐水-Ⅰ组及热盐水-Ⅱ组之间大鼠胃黏膜改变无统计学差异。盐水、热水灌胃至10wk,两组大鼠胃黏膜均未见萎缩?
OBJECTIVE: To establish a new method to rapidly establish an animal model of atrophic gastritis by using the saltiness and heat of food. Methods: Ninety-six healthy and sexually-mature (SD) SD rats aged 7 weeks were divided into 6 groups according to the principle of randomization: normal feeding group (normal feeding, drinking boiled water), normal control The rats in the saline group (25 ℃ 150g / L sodium chloride intragastrically once a day, each time 2.5mL), hot water group (55 ℃ Distilled water, once a day, every 2 / 5mL), hot saline - Ⅰ group (55 ℃ 150g / L sodium chloride intragastric administration, once daily, 2.5ml each) (55 ℃ 150g / L sodium chloride, 2 times a day, each 2.5ml). Each group of 16, using rack-cage, temperature 24 ± 1 ℃, humidity 50-60%. At the beginning of the experiment, four normal rats were sacrificed and the specimens of the stomach were collected for the control. After each 2wk each group executed 2, to observe mucosal atrophy, modeling success (shrinkage) that is to terminate the test, to retain gastric mucosa specimens. Tissue sections were blind read by hand. Atrophic gastritis standard formulation with reference to the 1994 Houston gastritis diagnostic classification criteria and 2000 Jinggangshan gastritis classification criteria, using a micrometer to mucosal thickness, gland width and cell thickness were measured. Statistical analysis: using t test. Results: The normal gastric mucosal surface was smooth and without erosion. The lamina propria had no infiltration of inflammatory cells, no submucosal edema and no inflammatory cell infiltration in the muscular layer. There was no significant difference in gastric mucosa between hot salt water-Ⅰ group and hot saline-Ⅱ group when the rats in hot salt water were given gavage for 10 weeks. Saline, hot water gavage to 10wk, gastric mucosa in both groups showed no atrophy?