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目的:观察骨髓基质细胞立体定向移植对大鼠脑缺血损伤后神经功能恢复的作用并探讨其作用机制。方法:制作SD大鼠大脑中动脉缺血模型(MCAO);体外培养骨髓基质细胞,观察其生物学特性以及立体定向移植后对脑缺血损伤后神经功能改善情况。结果:骨髓基质细胞体外可以长期传代、扩增,分泌NGF、VEGF等多种神经保护性因子;立体定向移植后,骨髓基质细胞在脑内存活、迁徙,小部分分化成具有神经元表面标志的细胞,与对照组相比,骨髓基质细胞移植组神经功能改善情况好于对照组。结论:骨髓基质细胞具有多向分化潜能,表达并分泌多种神经保护性营养因子。立体定向移植MSCs,对改善脑缺血损伤后的神经功能状况具有积极作用。
OBJECTIVE: To observe the effect of stereotactic transplantation of bone marrow stromal cells on neurological recovery after cerebral ischemia in rats and to explore its mechanism. Methods: The middle cerebral artery occlusion model (MCAO) was established in SD rats. BMSCs were cultured in vitro and their biological characteristics were observed. The neurological function of the MCAO rats after stereotactic transplantation was observed. RESULTS: BMSCs could be passaged, expanded, secreted and secreted NGF and VEGF in vitro for a long time. After stereotactic transplantation, BMSCs survived and migrated in the brain and differentiated into neurons with surface markers Compared with the control group, the neurological function of bone marrow stromal cell transplantation group was better than that of the control group. CONCLUSION: BMSCs possess multi-directional differentiation potential and express and secrete a variety of neuroprotective trophic factors. Stereotactic transplantation of MSCs has a positive effect on improving neurological status after cerebral ischemia.