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动用全外实验，对ＮＡＤＰＨ／ＦｅＳＯ４诱发的微粒体脂质过氧化（ＬＰ）发生条件及反应历程进行了探讨。结果表明：在３２７℃水浴，微粒体蛋白浓度为１．０ｍｇ／ｍｌ，ｐＨ值７．４的０．１ｍｏｌ／ＬＴｒｉｓ－ＨＣＬ缓冲液的条件下，１５０μｍｏｌ／ＬＮＡＤＰＨ和４．４５μｍｏｌ／ｌ／ＬＦｅＳＯ４可使微粒全中丙二醛（ＭＤＡ）含量于反应１５分钟内迅速上升，３０分钟时达到高峰，以后趋于平稳。揭示一定比例的ＮＡＤＰＨ／ＦｅＳＯ４可有效地激发微粒体ＬＰ。本研究初步建立子酶促微粒体ＬＰ模型
In this experiment, the conditions and reaction course of microsomal lipid peroxidation (LP) induced by NADPH / FeSO4 were discussed. The results showed that 150μmol / L NADPH and 4.45μmol / L / L FeSO4 could be obtained under the condition of 327 ℃ water bath, microsomal protein concentration 1.0mg / ml and pH 7.4 0.1mol / L Tris-HCL buffer The content of malondialdehyde (MDA) in the whole particles increased rapidly within 15 minutes and peaked at 30 minutes, then stabilized. Reveal a certain proportion of NADPH / FeSO4 can effectively stimulate microsomal LP. In this study, sub-enzymatic microsomal LP model was established