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目的:建立变换波长的方法测定复方樟脑搽剂中樟脑和苯酚的含量。方法:采用高效液相色谱法测定复方樟脑搽剂中樟脑和苯酚的含量,色谱柱Shim-pack VP-ODS(150 mm×4.6 mm,5μm),以甲醇-水(65∶35)为流动相,流速:1.0 mL/min,柱温:25℃,进样量:20μL,检测波长范围:0~5 min为270 nm,5~10 min为288 nm。结果:樟脑和苯酚的线性范围分别为0.051 40~1.028 00 mg/mL(r=0.999 9)与0.052 32~1.046 00 mg/mL(r=0.999 9),平均回收率分别为97.82%(RSD=0.9%)和98.09%(RSD=1.9%)。结论:本法操作简便、结果准确、重现性好,可用于复方樟脑搽剂的质量控制。
Objective: To establish a method for determination of camphor and phenol in compound camphor liniment by changing wavelength. Methods: The contents of camphor and phenol in compound camphor liniment were determined by high performance liquid chromatography. The mobile phase was Shim-pack VP-ODS (150 mm × 4.6 mm, 5 μm) with methanol-water (65:35) , Flow rate: 1.0 mL / min, column temperature: 25 ℃, injection volume: 20 μL, detection wavelength range: 270 nm for 0-5 min and 288 nm for 5-10 min. Results: The linear range of camphor and phenol were 0.051 40 ~ 1.028 00 mg / mL (r = 0.999 9) and 0.052 32 ~ 1.046 00 mg / mL (r = 0.999 9) .The average recoveries were 97.82% 0.9%) and 98.09% (RSD = 1.9%). Conclusion: This method is simple, accurate, reproducible and can be used for the quality control of compound camphor liniment.