【摘 要】
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Objective By hybridization in situ and biomechanical approach of platelet derived growth fator mRNA(PDGFmRNA) and insulin like growth factor mRNA(IGFmRNA),we di
【机 构】
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Second Hospital of Heibei Medical University,Shijiazhuang 050000,ChinaThird Hospital of Heibei Medic
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Objective By hybridization in situ and biomechanical approach of platelet derived growth fator mRNA(PDGFmRNA) and insulin like growth factor mRNA(IGFmRNA),we discussed the influence of the platelet concentrated liquid on the healing of rabbit ulna fracture.Method We selected 24 New Zealand rabbits,divided them into 4 groups randomly (blank group, serum control group, group with platelet concentrated liquid and group with bone graft and platelet concentrated liquid), and then made the fracture model on the middle of ulna which was fixed by finger armor plate.Before the operation, we drew out 6 ml blood from femoral artery, performed anti coagulation with the Sodium Citrate and centrifugated by low and the followed high speed.We purified the white blood plate and injected it into the fracture position.The rabbits were killed at 1st, 2nd, 4th and 6th week.Qualitative analysis by hybridization in situ of PDGFmRNA and IGFmRNA and biomechanical measurement on the 6th week sample were made.Result Bone callus could be seen on the radius specimen in various degrees when the rabbits were killed at 1st,2nd,4th and 6th week, particularly in the last week.The average maximum destructive load on the fracture tip is higher to the control, and there is significant difference(P< 0.01). Conclusion The local application of platelet concentration on the fracture tip can accelerate its healing.
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