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目前,人们对中枢神经系统的递质机制的认识依然相当肤浅。近年发展起来的脑脊髓活组织切片标本的电生理研究为深化这方面的知识开劈了一条重要途径。本研究利用新生(8—16天)大白鼠的脊髓胸腰段作横行薄切片(400μm厚)。以细胞内记录技术对切片内的前角运动神经元(MN)进行了突触电位的生理和药理学研究。结果表明,运动神经元可以用出现逆行动作电位来鉴定。在100个受记录的MN中,有10个细胞对腹根的刺激出现超极化反应。该反应可被低钙克
At present, people’s understanding of the neurotransmitter mechanism of the central nervous system is still rather superficial. In recent years, the development of brain spinal biopsy specimens of electrophysiological research to deepen the knowledge of this area open an important way. In this study, the thoracolumbar spinal cord of newborn (8-16 day) rats was used for transverse thin section (400μm thick). Physiological and pharmacological studies on the synaptic potential of anterior horn motoneurons (MN) in sections were performed using intracellular recording techniques. The results show that motor neurons can be identified by the presence of retrograde action potentials. Ten of the 100 recorded MNs hyperpolarized to the stimulation of the ventral roots. The reaction can be low calcium grams