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目的探讨2种不同破壁方法的玉米花粉对大鼠血脂代谢的影响。方法将大鼠随机分成3组,设1个高脂对照组、1个高脂+5.0g/kg.BW超临界CO2破壁玉米花粉组和1个高脂+5.0 g/kg.BW机械破壁玉米花粉组。自由进食、饮水,实验期8周,在实验3、6和8周末各测空腹TG、TC、HDL-C和LDL-C含量1次。实验末期,取血清测apoB、瘦素含量和各项血生化指标;断头处死,进行大体解剖观察,留取心脏、肝脏、脾脏、肾脏、睾丸、前列腺和主动脉称重、计算脏体比,做病理组织学检查,并测肝脂肪含量;每组随机取8只大鼠肝脏做超薄切片、电镜观察。结果 2个玉米花粉组大鼠TG、TC、LDL-C、apoB、GPT、GOT、肝脂肪含量、肝脏重、肝体比、前列腺重和前列腺体比均明显低于对照组(P<0.05或P<0.01);2个玉米花粉组大鼠肝脏的损伤和肝细胞肿胀、变性等病变程度明显较轻,肝组织病理变化总得分明显低于对照组(P<0.01)。结论 2种不同破壁方法的玉米花粉主要均通过明显拮抗高脂饮食大鼠apoB和LDL-C升高,促进机体脂质代谢,达到预防高脂饮食大鼠TG、TC和肝脂肪含量升高的作用,改善肝功能,减轻肝组织和肝细胞的损伤,从而降低高脂饮食大鼠脂质代谢紊乱和高脂血症的风险。2种不同破壁方法的玉米花粉尚均具有明显拮抗高脂饮食大鼠肝脏重、肝体比、前列腺重和前列腺体比升高的作用。2种不同破壁方法对玉米花粉调节血脂及其作用机制未见明显影响。
Objective To investigate the effects of two different methods of broken corn on blood lipid metabolism in rats. Methods The rats were randomly divided into three groups: one high fat control group, one high fat + 5.0g / kg.BW supercritical CO2 broken corn pollen group and one high fat +5.0 g / kg.BW mechanical broken Wall corn pollen group. Free to eat, water, the experimental period of 8 weeks, at the end of the experiment 3, 6 and 8 each measured fasting TG, TC, HDL-C and LDL-C content once. At the end of the experiment, serum apoB, leptin content and various blood biochemical indicators were taken; decapitation was executed, gross anatomy was observed, heart, liver, spleen, kidney, testis, prostate and aorta were weighed, , Histopathological examination, and measurement of liver fat content; each group of 8 rats were taken to do ultra-thin liver slices, electron microscopy. Results TG, TC, LDL-C, apoB, GPT, GOT, liver fat, liver weight, liver body weight, prostate weight and prostatic body weight in two corn pollen groups were significantly lower than those in control group (P <0.05 or P <0.01). The liver damage and liver cell swelling, degeneration and other pathological changes in the two corn pollen groups were significantly less than those in the control group (P <0.01). Conclusion The results showed that the two kinds of pollen-breaking methods mainly antagonized the increase of apoB and LDL-C in rats fed with high-fat diet and promoted lipid metabolism in the body to prevent the increase of TG, TC and hepatic fat in high-fat diet rats , Improve liver function, reduce the damage of liver tissue and liver cells, thereby reducing the risk of dyslipidemia and hyperlipidemia in rats with high fat diet. Two different methods of breaking the wall of corn pollen are still significantly antagonized the high-fat diet of liver weight, liver body ratio, prostate weight and prostate body ratio increased role. Two different methods of breaking wall of corn pollen to regulate blood lipids and its mechanism of action had no significant effect.