论文部分内容阅读
线粒体ATP合成酶是氧化磷酸化过程中ATP合成起关键作用的多亚基复合体,但近来发现它们在植物应对非生物胁迫反应中起着十分重要的作用。因此,对ATP合成酶的各亚基基因功能的研究有助于阐释其在植物逆境条件下的调节机制,并为研究植物抗逆和防卫反应提供新的途径。线粒体ATP合成酶是能量代谢的关键酶,参与氧化磷酸化反应。atpC基因所编码的线粒体ATP合成酶γ亚基是线粒体ATP合成酶的功能亚基。为了进一步研究它在胁迫反应中的功能,以小麦cDNA为模板,通过RT-PCR方法扩增出atpC基因。将该基因的cDNA编码序列连接到pCAMBIA1301中,成功构建了小麦pCAMBIA-atpC植物超量表达载体,为后续的转基因研究奠定了基础。
Mitochondrial ATP synthase is a multisubunit complex that plays a key role in ATP synthesis during oxidative phosphorylation, but recently they were found to play a very important role in plant responses to abiotic stresses. Therefore, the study of the function of each subunit gene of ATP synthase helps to explain its regulatory mechanism under stress conditions in plants, and provides a new way to study plant anti-retrogradation and defense responses. Mitochondrial ATP synthase is a key enzyme in energy metabolism and is involved in oxidative phosphorylation. Mitochondrial ATP synthase γ subunit encoded by atpC gene is a functional subunit of mitochondrial ATP synthase. In order to further study its function in the stress response, atpC gene was amplified by RT-PCR using wheat cDNA as a template. The cDNA coding sequence of this gene was ligated into pCAMBIA1301, and overexpression vector pCAMBIA-atpC was successfully constructed, which lays the foundation for the subsequent study of transgenic plants.