Establishment of cervical lymph node metastasis model of squamous cell carcinoma in the oral cavity

• 作 者: SUN Rui ZHANG Jian-gang GUO Ch
• 机 构: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University,
• 出 处: 中华医学杂志（英文版）
• 发表日期: 2008年19期
• 关键词: animal model lymphatic metastasis squamaus cell carcinoma mouth neoplasm

Background Oral squamous cell carcinoma (OSCC) is the most prevalent malignant tumor in the head and neck region,comprising more than 90% of all oral malignancies. A feasible approach for an animal model to study OSCC lymph node metastasis was established and biological behaviors of three oral squamous cell carcinoma cell lines were compared.Methods After implanting three kinds of call lines (GDC185, Tca8113, Tca83) into three different anatomical sites in nude mice, namely the tongue, floor of the mouth, and axillary fossa, we observed the tumorigenicity and the metastatic capacity, which was confirmed by histopathology under a surgical microscope.Results The animal model injected with GDC185 cells into the floor of the mouth had the highest rate of neck lymph node metastasis (55.6%) and the call lines had significantly different biological behaviors.Conclusions Nude mice injected with GDC185 cells into the floor of the mouth could be used as a feasible animal model to study neck metastasis of oral squamous cell carcinoma.