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采用ＰＣＲ技术直接从致病性钩端螺旋体（简称钩体）赖型０１７菌株基因组中扩增钩体外膜蛋白基因ＯｍｐＬ１，并克隆到大肠杆菌—卡介苗穿梭质粒载体ｐＹ６００２中，重组质粒经电转化导入卡介苗。斑点杂交筛选重组卡介苗，再通过免疫印迹对其表达进行初步研究。在所得６个重组卡介苗中，有３个表达了ＯｍｐＬ１基因产物，其中一个表达较强。该研究为发展新一代高效广谱的钩体基因工程疫苗打下了基础。
The OMPL1 gene was amplified directly from the genome of pathogenic Leptospira strain 017 by PCR and cloned into E. coli-BCG shuttle plasmid vector pY6002. The recombinant plasmid was electroporated into BCG. The recombinant BCG was screened by dot blot hybridization, and its expression was further studied by Western blotting. Of the six recombinant BCGs obtained, three expressed the OmpL1 gene product, one of which was strongly expressed. This research laid the foundation for the development of a new generation of highly efficient broadleaf genetically engineered vaccines.