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血清学试验在钩端螺旋体病的诊断中起着重要作用,但仅少数实验室有财力和专门技术进行显微镜凝集试验(MAT).因此需要一种快速简便的血清学试验对钩端螺旋体病进行早期诊断,以便及早实施有效的抗生素治疗.作者用市售间接血凝试剂盒(IHA)对107例病人的241份血样进行检测,并结合ELISA法和MAT对IHA加以评估.结果显示,107例病人中54例为钩端螺旋体病,53例为非钩端螺旋体病.IHA检出急性钩端螺旋体病的敏感性为100%,特异性为94%,阳性预测值为95%,阴性预测值为100%.用IHA对13份抗核抗体阳性血清、24份梅毒患者血清及16份性病研究所试验假阳性血清样本进行测定,结果显示均为阴性.IHA可测出人血清中的IgG和lgM抗体.从患者出现症状开始,IgM ELISA阴性结果的平均
Serological tests play an important role in the diagnosis of leptospirosis, but only a few laboratories have the financial and technical expertise to perform microscopic agglutination tests (MATs), so a quick and easy serological test is needed for leptospirosis Early diagnosis for effective early antibiotic therapy The authors tested 241 blood samples from 107 patients with a commercially available indirect hemagglutination kit (IHA) and assessed the IHA by ELISA and MAT.The results showed that 107 Of the patients, 54 were leptospirosis and 53 were non-leptospirosis.The detection of acute leptospirosis by IHA was 100% sensitive, 94% specific, and 95% positive predictive value Was 100% .IHA on 13 anti-nuclear antibody-positive sera, 24 syphilis serum and 16 sexually transmitted diseases Institute of false positive serum samples were determined, the results were negative.IHA can be measured in human serum IgG and lgM antibody. The mean IgM ELISA negative result from the patient’s onset of symptoms