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以体外培养的方法，对１５例骨髓增生异常综合征（ＭＤＳ）患者的骨髓，用补体介异的单克隆抗体选择性去除Ｔ细胞亚群，通过粒一单核祖细胞（ＣＦＵ－ＧＭ）集落产率的变化，观察Ｔ细胞亚群对造血细胞的抑制活性。结果发现１５例ＭＤＳ骨髓去除ＣＤ４细胞前后ＣＦＵ－ＧＭ集落产率无明显变化（Ｐ＞０．０５）；去除ＣＤ８细胞后，１１例ＭＤＳ骨髓ＣＦＵ－ＧＭ集落产率无明显变化（Ｐ＞０．０５），４例ＭＤＳ骨髓ＣＦＵ－ＧＭ集落产率增加３８％～７１％，与未去除ＣＤ８细胞组有明显差异（Ｐ＜０．００１）。以上结果提示，部分ＭＤＳ患者存在造血抑制世Ｔ细胞（ＨＩＴ），表明Ｔ细胞参与造血的调节过程。
In vitro culture of 15 patients with myelodysplastic syndrome (MDS) in patients with bone marrow, using a complement-specific monoclonal antibody selective removal of T-cell subsets, by a monocyte-derived mononuclear progenitor cells (CFU-GM) Yield changes observed T cell subsets of hematopoietic cells inhibitory activity. The results showed that CFU-GM colony yield did not change before and after removal of CD4 cells from 15 MDS bone marrow (P> 0.05). After CD8 cells were removed, there was no significant change in CFU-GM colony yield in 11 MDS patients (P> 0.05). 05). The yield of CFU-GM colonies increased by 38% -71% in 4 cases of MDS bone marrow, which was significantly different from that of the group without CD8 (P <0.001). These results suggest that some patients with MDS have hematopoietic suppression of T-cell (HIT), suggesting that T cells involved in the regulation of hematopoiesis.