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在模拟人体生理条件下,采用荧光光谱法及紫外-可见吸收光谱法研究了淫羊藿苷与牛血清白蛋白的相互作用。结果表明,淫羊藿苷对牛血清白蛋白有较强的猝灭作用,猝灭方式为静态猝灭。当温度为25℃和36℃时,淫羊藿苷对牛血清白蛋白的猝灭速率常数分别为4.37×10~(12)mol·L~(-1)·s~(-1)和3.90×10~(12)mol·L~(-1)·s~(-1),结合常数K_A为3.55×10~4L·mol~(-1)和3.97×10~4L·mol~(-1),结合位点数为1.12和1.04;根据Foerster非辐射能量转移理论,计算出淫羊藿苷与牛血清白蛋白之间的结合距离为2.08nm,热力学分析表明,淫羊藿苷与牛血清白蛋白之间以疏水作用力为主。
Under simulated human physiological conditions, the interaction between icariin and bovine serum albumin was studied by fluorescence spectroscopy and UV-Vis absorption spectroscopy. The results showed that icariin had a strong quenching effect on bovine serum albumin and the quenching mode was static quenching. The quenching rates of icariin to bovine serum albumin at temperature of 25 ℃ and 36 ℃ were 4.37 × 10-12 mol·L -1 s -1 and 3.90 respectively × 10-12 mol·L -1 s -1 and the binding constants K_A were 3.55 × 10 4 L · mol -1 and 3.97 × 10 4 L · mol -1 ), The number of binding sites was 1.12 and 1.04; According to the Foerster non-radiative energy transfer theory, the binding distance between icariin and bovine serum albumin was calculated to be 2.08 nm. Thermodynamic analysis showed that icariin and bovine serum albumin Hydrophobic interaction between the main protein.