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作者研究了含结核杆菌 38k Da蛋白的免疫刺激复合物 ( ISCOM)的最佳组成 ,分析了不同量的 N- (棕榈酰氧 )琥珀酰亚胺 ( PA-NHS,脂质尾端结合到蛋白质和 Quil A)对掺入 ISCOM的 38k Da蛋白免疫原性的影响。 作者按 5∶ 1和 1∶ 1的比例将 PA- NHS与 38k Da蛋白的氨基端连接 ,
The authors studied the optimal composition of an immunostimulatory complex (ISCOM) containing the Mycobacterium tuberculosis 38k Da protein and analyzed the effect of various amounts of N- (palmitoyloxy) succinimide (PA-NHS, the lipid tail bound to the protein And Quil A) on immunogenicity of 38 kDa protein incorporated into ISCOMs. The authors linked PA-NHS to the amino terminus of the 38k Da protein at a ratio of 5: 1 and 1: 1,