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目的 :研究环磷酰胺通过对雄大鼠的内分泌抑制和 /或诱导氧化应激作用而产生对睾丸雄激素和配子生成活性的毒性作用 ,同时评价维生素C的保护作用。方法 :以睾丸组织Δ5,3β 羟基甾醇脱氢酶 (HSD) ,17β -HSD ,过氧化物酶和过氧化氢酶活性 ,丙二醛 (MDA)及共轭双烯水平来评价睾丸的氧化应激 ;用免疫法测血浆睾酮 (T)水平 ,并在染色的睾丸切片 ,定量研究第VII生精周期时各种生殖细胞。结果 :环磷酰胺组的睾丸Δ53β HSD和 17β HSD活性显著抑制 ,血浆T水平降低、各种生殖细胞数目减少。同时 ,过氧化氢酶和过氧化物酶活性显著抑制 ,MDA和共轭双烯水平升高。用维生素C的共同使用可以使上述变化反转。结论 :所用剂量的环磷酰胺可引起睾丸配子形成和雄激素障碍 ,并导致睾丸氧化应激 ;维生素C的共同使用可反转这些作用
OBJECTIVE: To study the toxic effects of cyclophosphamide on the testosterone and gametogenic activity induced by endocrine inhibition and / or oxidative stress in male rats and to evaluate the protective effect of vitamin C. Methods: The oxidative stress of testis was evaluated by measuring the levels of Δ5,3β-hydroxysteroid dehydrogenase (HSD), 17β-HSD, peroxidase and catalase, malondialdehyde (MDA) and conjugated diene Plasma testosterone (T) levels were measured immunohistochemically and stained sections of testis were used to quantitatively study various germ cells during the seventh spermatogenic cycle. Results: The activity of Δ53β HSD and 17β HSD in the testis of cyclophosphamide group were significantly inhibited, the plasma T level decreased, and the number of various germ cells decreased. At the same time, catalase and peroxidase activities were significantly inhibited, MDA and conjugated diene levels increased. The combined use of vitamin C can make the above changes reversed. CONCLUSIONS: Cyclophosphamide at the dose used causes testicular gamete formation and androgen deprivation and leads to testicular oxidative stress; the co-use of vitamin C reverses these effects