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针对实验室进行藻毒素(MC)相关研究过程中MC样品昂贵且难以获取这一难题,以太湖水体中的蓝藻干藻粉为研究对象,分别以甲醇、乙醇、乙酸和丁醇-甲醇-水混合溶液为提取剂进行MC提取试验.考察了提取剂种类、干藻粉研磨时间、提取剂体积分数、搅拌时间、微波消解工艺条件对MC提取量的影响.结果表明:4种提取剂都可以提取MC-LR,提取量约为250～ 300 μg/g.而以甲醇为提取剂,研磨90 min,甲醇体积分数为60％,搅拌时间为2h,微波消解1 min时,MC-LR提取量最多约为300 μg/g左右.该方法可为实验室从事藻毒素毒性、化学或生物降解机制等研究工作提供便利,也可为藻泥的商品化提供参考.“,”Microcystin (MC-LR) samples are very expensive and difficult to obtain in experimental studies about MC.To solve this problem,the dry algae powder of blue-green algae collected from Taihu Lake is used to carry out experimental extraction of MC with methanol,ethanol,acetic acid and the solution of butanol and methanol as extracting agent respectively.Effects of extracting agent,grinding time of dry algae powder,concentration of extracting agent,stirring time and microwave digestion on the extraction of MC-LR are investigated.The resuhs show that MC-LR can be extracted from dry algae powder in the presence of four extracting agents,and the extraction amount is about 250-300 μg/g.The maximum extraction amount of MC-LR is up to 300 μg/g when methanol is used as extracting agent alone,grinding time is 90 min,methanol volume concentration being 60％,stirring time 2 h and microwave digestion 1 min.This method can provide convenience for the laboratory study on MC toxicity,chemical or biological degradation mechanism of MC-LR,and also provide reference for the commercial production of algae mud.