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用长臂光敏生物素标记含有马铃薯纺锤块茎类病毒（Ｐｏｔａｔｏｓｐｉｎｄｌｅｔｕｂｅｒｖｉｒｏｉｄ，ＰＳＴＶｄ）双拷贝ｃＤＮＡ的重组质粒ｐＳＴ－Ｂ１４，制备成光敏生物素标记ｃＤＮＡ探针，用核酸斑点杂交检测感染ＰＳＴＶｄ的马铃薯叶片和块茎提取物均出现较强的阳性杂交信号，而健康马铃薯为阴性．试验结果表明：光敏生物素标记ｃＤＮＡ探针检测感染ＰＳＴＶｄ的马铃薯叶片汁液最高稀释度为１１２８
The photo-biotin-labeled cDNA probe was prepared by using long-arm biotin-labeled recombinant plasmid pST-B14 containing the double copy cDNA of Potatospindletuberviroid (PSTVd) and the detection of potato leaves and tubers infected with PSTVd by dot blot hybridization Extracts showed strong positive hybridization signals, while healthy potato was negative. The results showed that the highest dilution of potato leaf juice infected with PSTVd detected by photo-biotin-labeled cDNA probe was 1128