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目的:建立HPLC-ELSD法同时测定平贝母中贝母素甲、贝母素乙含量的方法,并对市售样品进行考察,制定含量限度。方法:色谱柱:Waters C_(18)色谱柱(150mm×4.6mm,5μm),流动相:乙睛-二乙胺-水(70:0.03:30),流速:1.0 ml·min~(-1), ELSD参数:漂移管温度90℃,载气流速为2.2 L·min~(-1)。结果:贝母素甲、贝母素乙分别在0.06～0.35μg和0.08～0.50μg范围内线性关系良好。平均回收率(n=6)分别为98.4%,98.9%,RSD分别为1.8%,1.9%。结论:此法简便,快捷,结果可靠,可用于平贝母药材的质量评价,为今后其质量标准的修订提供了依据。
OBJECTIVE: To establish a HPLC-ELSD method for simultaneous determination of Fritillaria and Fritillaria B in Fritillaria ussuriensis Maxim. The commercial samples were investigated and the content limits were established. METHODS: The mobile phase consisted of a mobile phase of C 18 column (150 mm × 4.6 mm, 5 μm) with a mobile phase of acetonitrile: diethylamine at 70: 0.03: 30. The flow rate was 1.0 ml · min -1 ), ELSD parameters: drift tube temperature 90 ℃, carrier gas flow rate 2.2 L · min -1. Results: Fritillariae and peiminine B had a good linear relationship in the range of 0.06 ~ 0.35μg and 0.08 ~ 0.50μg respectively. The average recoveries (n = 6) were 98.4% and 98.9%, respectively, with RSDs of 1.8% and 1.9%, respectively. Conclusion: The method is simple, rapid and reliable. It can be used to evaluate the quality of Fritillaria cirrhosae and provide basis for the revision of its quality standard in the future.