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采用检测K－ras基因第12位密码子点突变方式（CGT、GTT、GAT）设计的顺序特异性引物（SSP），对胰腺癌及慢性胰腺炎的冰冻组织和胰液进行PCR扩增，产物借助常规电泳及染色判断有无K－ras基因突变及突变方式。结果：胰腺癌组织及胰液K－ras基因突变率分别为95．1％（39／41）、94．1％（16／17），而慢性胰腺炎组织（29例）、胰液（17例）及正常胰腺组织（12例）、胰液（4例）均无K－ras基因突变．研究表明：PCR－SSP快速、简便、特异、敏感，可以作为术前鉴别慢性胰腺炎和胰腺癌的一种实用新方法。
Sequential specific primers (SSP) designed to detect point mutations (CGT, GTT, GAT) in the 12th codon of the K-ras gene were used to amplify the frozen tissue and pancreatic juice from pancreatic cancer and chronic pancreatitis. Routine electrophoresis and staining were used to determine whether K-ras gene mutations and mutations occurred. RESULTS: The mutation rates of K-ras gene in pancreatic cancer tissue and pancreatic juice were 95.1% (39/41) and 94.1% (16/17), respectively, while chronic pancreatitis (29 cases) and pancreatic juice (17 cases). There were no K-ras mutations in normal pancreatic tissue (12 cases) and pancreatic juice (4 cases). Studies have shown that PCR-SSP is rapid, simple, specific, and sensitive and can be used as a practical new method to identify chronic pancreatitis and pancreatic cancer before surgery.