论文部分内容阅读
目的:建立并验证银杏叶提取物(GBE)血清药理学研究方法。方法:MTT法确定体系中血清添加量;HPLC法结合药效法确定大鼠给药方案及采血时间;以T-SOD、MDA为指标测定该含药血清的抗氧化能力。结果:大鼠按人临床用药等效剂量的5倍,每日两次连续4天灌胃给予GBE,于末次给药后2h采血时血清中GBE各有效成分的含量最高;体系中加入20%该条件下制备的含药血清对大鼠肾小球系膜细胞(MC)正常增殖没有影响;采用该方法制备的GBE含药血清具有明显的抗氧化能力。结论:本实验条件下制备的GBE含药血清具有明显的抗氧化能力,该血清药理学方法能用于GBE含药血清药效及机制研究。
Objective: To establish and validate the serum pharmacology of Ginkgo biloba extract (GBE). Methods: MTT method to determine the amount of serum added; HPLC method combined with pharmacodynamics to determine the rat dosing regimen and blood collection time; T-SOD, MDA as indicators of the drug-containing serum antioxidant capacity. Results: The rats were given 5 times the equivalent dose of human clinical medication twice a day for 4 consecutive days by intragastric administration of GBE, and the content of GBE in serum was the highest at 2 hours after the last administration. The system was supplemented with 20% The drug-containing serum prepared under these conditions had no effect on the normal proliferation of rat mesangial cells (MC). GBE-containing serum prepared by this method had obvious antioxidant capacity. Conclusion: GBE-containing serum prepared under the experimental conditions has obvious anti-oxidative capacity. The serum pharmacological method can be used to study the pharmacodynamics and mechanism of GBE-containing serum.