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为探明蒙古沙冬青根围丛枝菌根(arbuscular mycorrhiza,AM)和深色有隔内生真菌(dark septate endophytes,DSE)的空间分布,有效利用AM和DSE真菌资源,促进沙漠植被恢复和生态重建,选取内蒙古磴口、乌拉特后旗、阿拉善左旗和乌海4个样地,分别从蒙古沙冬青(Ammopiptanthus mongolicus)根围0~10cm、10~20cm、20~30cm、30~40cm和40~50cm 5个土层采集土样,研究了AM和DSE真菌的定殖和分布规律。结果表明:沙冬青根系能被AM真菌高度侵染成I-型(Intermediate type)丛枝菌根,并与DSE形成良好的共生关系,样地间AM真菌定殖率普遍高于DSE。乌拉特后旗DSE定殖率和孢子密度高于其他样地,孢子密度在4个样地随土层加深而降低,最大值均在0~10cm土层,AM真菌泡囊最高定殖率出现在0~20cm土层。DSE定殖率除磴口10~20cm土层显著高于40~50cm土层外,其他样地土层间均无显著差异。AM真菌孢子密度与土壤有机质、碱解氮、脲酶、酸性磷酸酶和碱性磷酸酶极显著正相关;AM真菌泡囊定殖率与碱性磷酸酶、脲酶和酸性磷酸酶显著负相关;AM真菌丛枝定殖率与脲酶和酸性磷酸酶显著负相关;AM真菌菌丝定殖率除与速效磷呈正相关外,AM真菌菌丝和DSE定殖率与其他土壤因子均无显著相关性。经通径分析,土壤有机质直接影响DSE定殖率,碱解氮、速效磷间接影响DSE定殖率。
In order to explore the spatial distribution of arbuscular mycorrhiza (AM) and dark septate endophytes (DSE) in the provenance of A. mongolica, AM and DSE fungi resources were effectively used to promote the recovery of desert vegetation and According to the ecological reconstruction, four plots of Ammopiptanthus mongolicus from Inner Mongolia were selected, including 0 ~ 10cm, 10 ~ 20cm, 20 ~ 30cm, 30 ~ 40 cm soil layer and 40 ~ 50 cm soil layer were collected. The colonization and distribution of AM and DSE fungi were studied. The results showed that A. sojae could be highly infested with AM fungi to form an arbuscular mycorrhizal fungi, which formed a symbiotic relationship with DSE. The colonization rate of AM fungi was generally higher than that of DSE. The colonization rate and spore density of DSE in Wulate Houqi were higher than those in other plots. The density of spore densities decreased with the deepening of soil depth in four plots, and the maximum values were in 0-10 cm soil layer. The highest colonization rate of AM fungal vesicles appeared In 0 ~ 20cm soil layer. The rate of DSE colonization was not significantly different from that of other plots except for the 10-20 cm soil layer at the mouth of the soil layer, which was significantly higher than that of the 40-50 cm soil layer. AM fungal spore density was significantly and positively correlated with soil organic matter, alkaline hydrolysis nitrogen, urease, acid phosphatase and alkaline phosphatase; AM fungal vesicle colonization rate was negatively correlated with alkaline phosphatase, urease and acid phosphatase; AM There was a significant negative correlation between the colonization rate of fungi and urease and acid phosphatase. The colonization rate of AM fungi had no significant correlation with other soil factors except for the positive correlation with AM. Through the path analysis, soil organic matter directly affects the DSE colonization rate, alkali hydrolysis of nitrogen, available phosphorus indirectly affect DSE colonization rate.