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利用以籼稻(Oryza sativa ssp.indica)品种特青为遗传背景的云南元江普通野生稻(O.rufipogon Griff.)(简称元江普野)渗入系为材料,采用单标记回归分析和渗入片段叠代法,对出糙率、整精米率、垩白粒率、垩白度、长宽比等5个品质性状的QTL进行了分析,初步定位了16个QTL,有10个QTL来自野生稻的等位基因能改良群体的品质性状。在第5染色体RM598附近检测到了同时影响长宽比和垩白粒率的QTL,来自野生稻的等位基因能增加长宽比和降低垩白粒率,贡献率也较高。在第8染色体RM152附近检测到降低垩白粒率和垩白度的QTL,其贡献率分别为14%和9%。研究结果不仅为品质性状分子标记辅助选择提供参考,而且充分显示了利用野生稻的优异基因改良栽培稻品质性状的巨大潜力。
By using the introgressing line of O. rufipogon Griff. (Oryza sativa ssp. Indica) as the genetic background in Yunnan Province, we used single-marker regression analysis and infiltration of fragments Method was used to analyze the QTLs of five quality traits such as roughness, milled rice rate, chalky grain rate, chalkiness, aspect ratio, etc. The QTLs were initially mapped for 16 QTLs and 10 QTLs for wild rice Genes can improve the quality of the population traits. QTLs affecting both aspect ratio and chalky grain rate were detected around RM598 on chromosome 5, and the alleles from wild rice increased the aspect ratio and decreased the chalky grain rate, with a higher contribution rate. QTLs for reducing chalky grain rate and chalkiness were detected around RM152 on chromosome 8, accounting for 14% and 9%, respectively. The results not only provide references for molecular marker-assisted selection of quality traits, but also fully demonstrate the great potential of using the excellent genes of wild rice to improve the quality traits of cultivated rice.