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目的:探讨中电导钙激活钾离子通道(SK4)蛋白与甲状腺乳头状癌中央区淋巴结转移的相关性。方法:分析88例甲状腺乳头状癌患者的组织芯片，根据SK4免疫组织化学染色结果分为SK4阳性表达组(55例)和SK4阴性表达组(33例)，细胞实验小干扰RNA(siRNA)敲除SK4的甲状腺乳头状癌细胞为实验组，甲状腺乳头状癌细胞为对照组。采用n χ2检验分析SK4表达与临床病理特征之间的关系，采用Logistic回归模型分析甲状腺乳头状癌中央区淋巴结转移危险因素。通过Transwell细胞迁移和侵袭实验验证SK4对甲状腺乳头状癌细胞侵袭转移的作用。采用SPSS 22.0(IBM)和Graphpad prism 5.0软件分析。应用n χ2检验和Fisher精确概率法分析组间差异，建立Logistic二元回归模型进行单因素、多因素分析。n 结果:88例甲状腺乳头状癌组织中SK4阳性者为55例，阳性率为62.5%。SK4在甲状腺乳头状癌中央区淋巴结转移中阳性表达率为73.3%(33/45)，SK4在各临床分期中阳性表达率为，Ⅰ期66.7%(24/33)，Ⅱ期23.1%(3/13)，Ⅲ期80.6%(25/33)，Ⅳ期50.0%(3/6)，n χ2检验结果显示SK4的表达水平与甲状腺乳头状癌中央区淋巴结转移、临床分期之间差异有统计学意义(n χ2＝4.611、11.762，n P0.05)。Logistic回归分析结果显示SK4阳性和临床分期，是甲状腺乳头状癌中央区淋巴结转移的独立危险因素(n OR＝3.827、3.882，n P0.05), T-stage (n χ2=2.799, n P>0.05), and tumor site (n χ2=0.637, n P>0.05). Logistic regression analysis showed SK4 and clinical stage were independent risk factors for central lymph node metastasis of papillary thyroid carcinoma (n OR=3.827, 3.882, n P<0.05). After SK4 knockout, the invasion and migration ability of papillary thyroid cancer cells was obviously inhibited. The invasion and migration capacity in the SK4 knockout group was significantly lower than in the control group (224 cells vs. 690 cells; 196 cells vs. 571 cells;n t=185.900, 138.600, n P<0.01).n Conclusion:SK4 expression is strongly linked to central lymph node metastasis of papillary thyroid cardnoma.