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在动情周期和假孕过程中,大鼠子宫合成前列腺素的能力发生变化。作者观察了在假孕及妊娠早期大鼠子宫在离体条件下前列腺素的产生以及消炎痛对大鼠着床及妊娠的影响。将雌性大白鼠分为六组进行实验:一组大鼠用假交配造成假孕,于假孕第四至六天,每天将4只大鼠在轻度乙醚麻醉下自心脏取血,抗凝、离心取得血浆做孕酮测定。大鼠处死后立即取下子宫,称重,放于15ml Kred′s液中匀浆,在37℃孵育60分钟,然后提取前列腺素。用放射免疫分析法分别测定6-氧-PGF_(1α),PGE_2及PGF_(2α)。6-氧-PGF_(1α)也用气相色谱-质谱测定。二组大鼠与雄鼠合笼后
During the estrus cycle and the process of pseudopregnancy, the ability of rat uterus to synthesize prostaglandins changes. The authors observed the effects of prostaglandin production in vitro on pseudohypuppression and early pregnancy in rat uterus and indomethacin on implantation and pregnancy in rats. The female rats were divided into six groups for experiment: One group of rats were given false pregnancy with fake mating. Four to six days of pseudopregnancy, four rats were perfused with blood from the heart under mild ether anesthesia. , Obtained by centrifugation plasma progesterone determination. Immediately after sacrifice, the uterus was removed, weighed, homogenized in 15 ml of Kred’s solution, incubated at 37 ° C for 60 minutes, and then prostaglandin was extracted. 6-Oxygen-PGF_ (1α), PGE_2 and PGF_ (2α) were determined by radioimmunoassay. 6-Oxygen-PGF_ (1α) was also determined by gas chromatography-mass spectrometry. Two groups of rats and males cage after