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目的:研究趋化素样因子1(CKLF-1)在哮喘大鼠模型肺组织中的表达与定位。方法:将20只健康雄性SD大鼠随机分为哮喘模型组和正常对照组,哮喘组以卵清蛋白(OVA)作为过敏原、氢氧化铝为佐剂致敏SD大鼠后,雾化吸入5%OVA激发制备哮喘大鼠模型,观察大鼠行为学改变。到末次激发后24h处死两组大鼠,做肺泡灌洗后进行细胞计数、分类计数;并采用免疫组化方法检测CKLF-1在大鼠肺组织中的表达及定位。结果:哮喘模型组大鼠在吸入5%OVA激发后表现为精神差、反应迟钝、呼吸急促,伴有腹肌抽动,正常对照组除轻微呼吸加快外,无其他反应;哮喘模型组肺泡灌洗液中白细胞总数、中性粒细胞数及嗜酸性粒细胞数均明显高于正常对照组(P<0.05,P<0.01),说明造模成功。哮喘模型组大鼠肺组织中CKLF-1的表达明显高于正常对照组,且在气管上皮细胞及其周围的炎性细胞、肺泡巨噬细胞中定位。结论:CKLF-1在哮喘大鼠肺组织中的表达明显升高,提示CKLF-1与哮喘的发作密切相关。
AIM: To investigate the expression and localization of CKLF-1 in lung tissue of asthmatic rats. Methods: Twenty healthy male Sprague-Dawley rats were randomly divided into asthma model group and normal control group. Ovalbumin (OVA) was used as an allergen in asthmatic group. SD rats were sensitized with aluminum hydroxide as adjuvant, 5% OVA stimulated asthma rat model was established to observe the behavioral changes in rats. Rats in the two groups were sacrificed 24 h after the last challenge. After alveolar lavage, the cells were counted and classified. The expression and localization of CKLF-1 in lung tissue were detected by immunohistochemistry. Results: The asthmatic model group showed poor mental performance, slow response, shortness of breath and abdominal twitch after 5% OVA challenge. There was no other reaction in the normal control group except for slight respiration; in the asthma model group, the alveolar lavage The total number of white blood cells, neutrophils and eosinophils in the fluid were significantly higher than those in the normal control group (P <0.05, P <0.01), indicating successful modeling. The expression of CKLF-1 in the lung tissue of asthmatic model group was significantly higher than that of the normal control group, and located in the tracheal epithelial cells and surrounding inflammatory cells, alveolar macrophages. Conclusion: The expression of CKLF-1 in the lung tissue of asthmatic rats was significantly increased, suggesting that CKLF-1 is closely related to the onset of asthma.