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用放射配体受体结合试验法，研究了新化合物三环哌酯与人大脑皮质Ｍ受体的结合特性，并与ＱＮＢ作了比较。饱和实验结果显示，［３Ｈ］三环哌酯的结合参数与［３Ｈ］ＱＮＢ相近，两种配体的作用均符合单位点模型。竞争性抑制实验结果表明二者作用强度相当。［３Ｈ］三环哌酯的结合和解离速率常数均较［３Ｈ］ＱＮＢ大，且其与皮质Ｍ受体的解离受季铵酚的变构调节，结果提示，两种配体与Ｍ受体有一些不同的结合特性，在Ｍ受体研究中，［３Ｈ］三环哌酯可以作为［３Ｈ］ＱＮＢ的补充工具
Radioligand receptor binding assay was used to study the binding properties of tricyclic piperazine ester to M receptor in human cerebral cortex and compared with QNB. The results of saturation experiments showed that the binding parameters of [3H] tricyclohexyl ester were similar to that of [3H] QNB. Both ligands were in agreement with the single site model. Competitive inhibition test results show that both the role of intensity. The binding and dissociation rate constants of [3H] tricyclohexyl ester are larger than that of [3H] QNB, and their dissociation with cortical M receptor is regulated by the allosteric action of quaternary ammonium. The results suggest that both ligands and M receptor There are some different binding properties of the [3H] tricyclohexyl ester as an additional tool for [3H] QNB in M receptor studies