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Objective:To select the specific aptamer of carcinoembryonic antigen (CEA),one of the most attractive molecule for cancer target therapy and imaging.Methods:Seven rounds in vitro selection were performed against the purified CEA protein.Ligand-mediated target purification and Co-immunoprecipitation were adopted to verify the specific binding of the aptamer to the purified and native protein separately.Results:The CEA-specific aptamer which can bind both the purified and native protein with the high specificity was obtained.Conclusion:This is the first time the CEA specific apatmer was produced.The results in this study provides the preliminary evidence for further investigation and application of CEA-aptamer in the future.
Objective: To select the specific aptamer of carcinoembryonic antigen (CEA), one of the most attractive molecule for cancer target therapy and imaging. Methods: Seven rounds in vitro selection were performed against the purified CEA protein. Ligand-mediated target purification and Co- immunoprecipitation were adopted to verify the specific binding of the aptamer to the purified and native protein separately. Results: The CEA-specific aptamer which can bind both the purified and native protein with the high specificity was obtained. Confluence: This is the first time the CEA specific apatmer was produced. The result in this study provides the preliminary evidence for further investigation and application of CEA-aptamer in the future.