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AIM:To study the inhibitory effect of transfected PTEN onLoVo cells.METHODS:Human PTEN cDNA was transferred into LoVocells via lipofectin and PTEN mRNA levels and its expressionwere analyzed by Western blot and flow cytometry.Beforeor after transfection,the effects of 5-Fu on inhibiting cellproliferation and inducing apoptosis were measured byflow cytometry,DNA bands and MTF.RESULTS:PTEN transfection significantly up-regulated PTENexpression in LoVo cells.5-Fu inhibited cell proliferationand induced apoptosis in transfected LoVo cells.CONCLUSION:Transfected PTEN can remarkably up-regulatePTEN expression in LoVo cells and promote the apoptosis.PTEN transfection is associated with 5-Fu treatment effectand has a cooperatively cytotoxic effect.
AIM: To study the inhibitory effect of transfected PTEN onLoVo cells. METHODS: Human PTEN cDNA was transferred into LoVocells via lipofectin and PTEN mRNA levels and its expression were analyzed by Western blot and flow cytometry. Beforeforefection after transfection, the effects of 5-Fu on Inhibitory cell proliferation and inducing apoptosis were measured by flow cytometry, DNA bands and MTF. RESULTS: PTEN transfection significantly up-regulated PTENexpression in LoVo cells. 5-Fu inhibited cell proliferation and induced apoptosis in transfected LoVo cells. CONCLUSION: Transfected PTEN can remarkably up-regulate PTEN expression in LoVo cells and promote the apoptosis. PTEN transfection is associated with 5-Fu treatment effect and has a cooperatively cytotoxic effect.