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环氧乙烷在工业及医学卫生方面应用广泛,对其毒性研究报道不少。动物实验表明环氧乙烷具有致突变性,为进一步了解其对人体细胞的影响,我们对某厂环氧乙烷车间工人进行了外周血淋巴细胞染色体畸变(CA)与姐妹染色单体交换(SCE)研究。 1 对象与方法 1.1 对象 环氧乙烷车间工人26名,其中男20名,女6名,年龄26～47岁,工龄4～14年;非接触环氧乙烷者29人,作为正常对照,年龄及工龄等类同接触组。 1.2 方法 CA标本的制作:取肝素抗凝血加入含RPMI1640完全培养基的瓶内,37℃培养72h,终止前加入秋水仙素,常规制片,吉姆萨染色,每份分析50个分裂相,45条染色体以下者不予统计,计算每百个
Ethylene oxide is widely used in industrial and medical hygiene, and many studies on its toxicity have been reported. Animal experiments show that ethylene oxide has mutagenicity, in order to further understand its impact on human cells, we conducted a workshop ethylene oxide plant workers were peripheral blood lymphocyte chromosome aberrations (CA) and sister chromatid exchange SCE) study. 1 Subjects and Methods 1.1 Object Twenty-six ethylene oxide workers, including 20 males and 6 females, aged 26 to 47 years old, working for 4 to 14 years; non-contact ethylene oxide were 29 people, as a normal control, Age and seniority and other similar contact group. 1.2 Methods CA sample preparation: take heparin anticoagulant added RPMI1640 complete medium containing bottles, 37 ℃ for 72h, before the end of adding colchicine, conventional production, Giemsa staining, each analysis of 50 split phase, The following 45 chromosomes are not counted, calculated per 100